NU 7026

SKU: orb1305492

Description

NU 7026

Research Area

Cell Biology, Molecular Biology, Signal Transduction

Key Properties

• CAS Number: 154447-35-5
• MW: 281.31
• Purity: 99.96%
• Formula: C₁₇H₁₅NO₃
• SMILES: O=C1C=2C(=C3C(=CC2)C=CC=C3)OC(=C1)N4CCOCC4
• Target: Apoptosis,ATM/ATR,PI3K,DNA-PK
• Solubility: DMSO:0.7 mg/mL (2.49 mM)

Bioactivity

• Target IC50:
  DNA-PK:0.23 μM|PI3K:13 μM
• In Vivo:
  NU7026 inhibits DNA DSB repair in the V3YAC cell line by 56%. In the CLL cell line (I83) and primary CLL lymphocytes, NU7026 exhibits synergistic cytotoxic activity with Bendamustine at concentrations below 10 μM. In K562 cells, NU7026 (10 μM) enhances the growth inhibitory effects of doxorubicin, amsacrine, idarubicin, aminobenzotriazole, etoposide, and mitoxantrone, with PF50 values ranging approximately from 19 (for mAMSA) to 2 (for idarubicin). Also, at 10 μM, NU7026 enhances the growth inhibition induced by etoposide in leukemia cells (PF50: 10.53) and the etoposide-induced G2 phase cell cycle arrest in K562 cells. A 4-hour exposure to NU7026 (10 μM) combined with 3 Gy radiation is necessary for a significant radiosensitization effect in CH1 human ovarian cancer cells. In the I83 cell line, NU7026 (10 μM) increases G(2)/M phase arrest and γH2AX throughout the cell cycle, both induced by Bendamustine, as well as apoptosis. Finally, at 55 μM, NU7026 notably induces telomere fusion in p53-deficient MEFs and causes less telomere fusion in MEFs lacking both p53 and Ligase IV.
• In Vitro:
  The bioavailability of NU7026, administered intraperitoneally (i.p.) or orally (p.o.) at a dose of 20 mg/kg, was found to be 20% and 15%, respectively. In mice, NU7026 administered intravenously (i.v.) at the same dosage exhibited a rapid plasma clearance rate of 0.108/h.
• Cell Research:
  NU7026 is dissolved in DMSO and stored, and then diluted with appropriate media before use. I83 cells are plated in RPMI 1640 medium with 10% FBS (1.5×105 cells/mL) and treated with vehicle (DMSO), 5 μM CLB, CLB IC50, 10 μM NU7026, or the combination of both drugs for 0, 6, 24, and 48 h. Cell cycle distribution, apoptosis, DNA-PK phosphorylation, and γH2AX determination are determined, and they are expressed as a percentage of cells in each phase of the cycle. DNA content is analyzed with a FACSCalibur flow cytometer equipped with CellQuest software.

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Inhibitor, NU 7026, NU7026, NU-7026, LY 293646, LY293646, LY-293646, inhibit, Apoptosis, ATR, ATM, DNAPK, DNA-dependent protein kinase, DNA-PK Inhibitor II, DNA-PK, PI3K

Compound Identifiers

PubChem CID

9860529