NSC59984

SKU: orb1300354

Description

NSC59984 is a small molecule activator of the p53 pathway that promotes the degradation of mutant p53 proteins and activates p73. It is used in cancer research to study p53 family biology and has demonstrated activity in both cellular and animal models of tumorigenesis.

Research Area

Cell Biology

Key Properties

• CAS Number: 803647-40-7
• MW: 265.27
• Purity: 99.59% (May vary between batches)
• Formula: C₁₂H₁₅N₃O₄
• SMILES: CN1CCN(CC1)C(=O)\C=C\c1ccc(o1)[N+]([O-])=O
• Target: MDM-2/p53,p53
• Solubility: Ethanol:49 mg/mL (184.72 mM);H2O:< 1 mg/mL (insoluble or slightly soluble);DMSO:55 mg/mL (207.34 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (7.54 mM)

Bioactivity

• In Vivo:
  In nude mice bearing DLD-1 xenograft tumors, NSC59984 (45 mg/kg, i.p.) significantly represses tumor growth in a p73-dependent manner.
• In Vitro:
  NSC59984 selectively reactivates the p53 pathway and induces p73-dependent cell death in mutant p53-expressing human colorectal cancer cells. It also promotes cell death in various cancer cell lines with different p53 mutations, showing minimal genotoxicity in normal cells.
• Cell Research:
  Cells are seeded at 5,000 cells per well on 96-well plates. Cells are mixed with an equal volume of CellTiter-Glo reagents, following the manufacturer's protocol, and bioluminescence imaging is measured using the IVIS imager.(Only for Reference)

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

NSC 59984, p53, NSC59984, NSC-59984, Inhibitor, inhibit, MDM-2/p53

Compound Identifiers

PubChem CID

5356520