NSC348884

SKU: orb1221760

Description

NSC348884 is a nucleophosmin inhibitor. NSC348884 also disrupts oligomer formation and induces apoptosis in human cancer cells.

Key Properties

• CAS Number: 81624-55-7
• MW: 636.81
• Purity: >98% (HPLC)
• Formula: C₃₈H₄₀N₁₀
• SMILES: Cc1cc2c(cc1)nc(CN(CCN(Cc1nc3c([nH]1)cc(C)cc3)Cc1nc3c([nH]1)cc(C)cc3)Cc1nc3c([nH]1)cc(C)cc3)[nH]2
• Target: nucleophosmin
• Solubility: DMSO : ≥ 34 mg/mL; 53.39 mM

Bioactivity

• In Vivo:
  NSC348884 (50 μg; i.p. once) reduces tumor growth. Animal model: C57Bl/6J mice with MOSEC/Luc cells injection. Dosage: 50 μg. Administration: Intraperitoneal injection; 50 μg once. Result: Reduced tumorigenesis, but not completely inhibited.
• In Vitro:
  NSC348884 (0-6 μM; 24 h) binds with NPM and disrupts NPM oligomer formation. NSC348884 (0.01-100 μM; 4 d) shows cytotoxicity to cancer cells. NSC348884 (1 μM; 4 d) shows cytotoxicity to mantle cell lymphoma with 0.1 nM doxorubicin. NSC348884 (1-5 μM; 24 h) induces cell apoptosis. NSC348884 (0-5 μM; 0-24 h) upregulates the concentration of p53.Cell Viability Assay Cell line: LNCaP and Granta cell lines. Concentration: 0.01-100 μM Incubation time: 4 days. Result: Inhibited LNCaP cells and Granta cell lines with IC50s of 4 and 1.7 μM, respectively. Apoptosis Analysis Cell line: LNCaP cell line. Concentration: 1-5 μM. Incubation time: 24 h. Result: Induced apoptosis in LNCaP cell line. Western blot analysis. Cell line: LNCaP cell line. Concentration: 0-5 μM. Incubation time: 0-24 h. Result: Dose-dependently increased both p53 protein level and phosphorylation on Ser15, and increased the concentration of p21. Western blot analysis. Cell line: SKOV3, ES2 and MOSEC/Luc cell lines. Concentration: 0, 2.5 and 5 μM Incubation time: 48 h. Result: Increased PD-L1 expression in ovarian cancer cells.

Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

NSC348884 | NSC 348884 | NSC-348884

Quick Database Links

Gene Symbol

nucleophosmin