NSC23005 Sodium

SKU: orb1304025

Description

NSC23005 Sodium

Research Area

Cell Biology

Key Properties

• CAS Number: 1796596-46-7
• MW: 305.32
• Purity: 99.71%
• Formula: C₁₃H₁₆NNaO₄S
• SMILES: C1CCC(CC1)NS(=O)(=O)c1ccc(cc1)C(=O)[O-].[Na+]
• Target: CDK,Others
• Solubility: DMSO:60 mg/mL (196.52 mM)

Bioactivity

• Target IC50:
  p18INK4C:5.21 nM (ED50)
• In Vivo:
  NSC23005 sodium selectively promotes HSC division by inhibiting p18, thereby activating CDK4/6. This novel and effective p18 inhibitor enhances HSC expansion In both murine and human models without significant cytotoxicity toward HSCs, and it does not augment leukemia cell proliferation.
• In Vitro:
  NSC23005 sodium is a novel class of INK4C (p18INK4C or p18) s All molecule inhibitor (p18SMIs), which is initially found by in silico 3D screening. NSC23005 sodium show the most potent bioactivity in hematopoietic stem cells (HSCs) expansion (ED5 = .21 nM). Notably, NSC23005 sodium does not show significant cytotoxicity toward 32D cells or HSCs, nor does it augment leukemia cell proliferation. NSC23005 sodium (ED5 = .21 nM), shows no activity in promotin the proliferation of leukemia cells.
• Cell Research:
  NSC23005 sodium is dissolved in DMSO and stored, and then diluted with appropriate medium before us. c-Kit enriched bone marrow (BM) cells are cultured for 5 days with cytokine combination plus NSC23005 sodium or DMSO. As positive controls, Primary uncultured bone marrow cells are treated by ultraviolet radiation (UV) for 10 minutes prior to the staining process for apoptosis analysis. Apoptosis and cell death are measured by AnnexinV and DAPI staining in the Annexin V-FITC Apoptosis Detection Kit. Apoptosis is measured on an FACS analyzer the data is analyzed using FlowJo softwar.

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Disclaimer: For research use only

Alternative Names

p18INK4C, Inhibitor, inhibit, NSC 23005, NSC23005, NSC-23005, NSC23005 Sodium, NSC-23005 Sodium

Compound Identifiers

PubChem CID

122705986