NSC95397

SKU: orb1300095

Description

NSC 95397 is a potent small-molecule inhibitor of Cdc25 dual-specificity phosphatases, with Ki values in the nanomolar range for Cdc25A, B, and C. It is widely used in biochemical and cell-based research to study cell cycle regulation, proliferation, and as a potential anticancer agent in experimental models.

Research Area

Cell Biology

Key Properties

• CAS Number: 93718-83-3
• MW: 310.39
• Purity: >98%
• Formula: C₁₄H₁₄O₄S₂
• SMILES: OCCSC1=C(SCCO)C(=O)c2ccccc2C1=O
• Target: Phosphatase
• Solubility: Soluble in DMSO (up to 15 mg/ml) or in Ethanol (up to 1 mg/ml).

Bioactivity

• Target IC50:
  CDC25A:32nM(ki)|CDC25B:96nM(ki)|CDC25C:40 nM(ki)
• In Vitro:
  NSC 95397 reduced cell viability and anchorage-independent growth of all the three colon cancer cell lines through inhibited proliferation and induced apoptosis via regulating cell-cycle-related proteins, including p21, cyclin-dependent kinases, and caspases.
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• Cell Research:
  To measure the cell proliferation activity of NSC 95397 against colon cancer cells, SW480 (1 × 10^4), SW620 (2 × 10^4), and DLD-1 (1 × 10^4) cells were seeded into 96-well plates. After overnight culture, NSC 95397 cells were added at the indicated concentrations. After 24 h of incubation, cell proliferation was determined in vitro using a BrdU cell proliferation assay kit. O.D. 450 values were analyzed by using a Multiskan PC.The apoptosis of colon cancer cells was determined by a PE Annexin V Apoptosis Detection Kit with 7-AAD. Briefly, cells were treated with the indicated concentration of NSC 95397 for 24 h. After 24 h, Cells were harvested and stained with PE Annexin V/7-AAD for 15 min. The stained cells were analyzed using FACSCanto II low cytometer and FCS Express software
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Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Phosphatase, Apoptosis, NSC 95397, inhibit, NSC95397, NSC-95397, Inhibitor

Compound Identifiers

PubChem CID

262093