NSC 65809

SKU: orb1298820

Description

NSC-65809, also known as Raphin1, is a selective small molecule inhibitor of the regulatory subunit R15B of protein phosphatase 1 (PP1). This tool compound is used in biochemical and cellular research to investigate PP1-mediated signaling pathways in both in vitro and in vivo experimental models.

Research Area

Metabolism Research

Key Properties

• CAS Number: 94023-67-3
• MW: 231.08
• Purity: 99.84% (May vary between batches)
• Formula: C₈H₈Cl₂N₄
• SMILES: C(=NNC(=N)N)C1=C(Cl)C(Cl)=CC=C1
• Target: Phosphatase
• Solubility: DMSO:35 mg/mL (151.46 mM)

Bioactivity

• In Vivo:
  Raphin1 was orally bioavailable, crossed the blood-brain barrier, and demonstrated efficacy in a mouse model of Huntington's disease.
• In Vitro:
  In cells, Raphin1 caused a rapid and transient accumulation of its phosphorylated substrate, resulting in a transient attenuation of protein synthesis. In vitro, Raphin1 inhibits the recombinant R15B-PP1c holoenzyme, but not the closely related R15A-PP1c, by interfering with substrate recruitment

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Raphin1, phosphatase PPP1R15B (R15B), NSC65809, NSC-65809, NSC 65809, 3-[(2,3-dichlorophenyl)methylene]carbazamidine

Compound Identifiers

PubChem CID

96297