NSC405020

SKU: orb1307234

Description

NSC 405020 is a small molecule that acts as a direct, noncatalytic inhibitor of membrane type-1 matrix metalloproteinase (MT1-MMP). It is utilized in biochemical and cell-based research to study MT1-MMP's role in processes such as extracellular matrix remodeling, cell migration, and tumor invasion in both in vitro and in vivo experimental models.

Research Area

Neuroscience

Key Properties

• CAS Number: 7497-07-6
• MW: 260.15
• Purity: >98%
• Formula: C₁₂H₁₅NCl₂O
• SMILES: CCCC(C)NC(=O)C1=CC=C(Cl)C(Cl)=C1
• Target: Protease
• Solubility: Soluble in DMSO (up to 25 mg/ml) or in Ethanol (up to 25 mg/ml).

Bioactivity

• In Vivo:
  NSC 405020 (0.5 mg/kg, intratumoral injection) significantly represses tumor growth. NSC 405020 causes a fibrotic, ΔPEX-like tumor phenotype and increases the level of COL-I.
• In Vitro:
  NSC 405020 is a noncatalytic inhibitor of MT1-MMP, directly interacts with PEX domain of MT1-MMP. NSC 405020 affects PEX homodimerization but not catalytic activity of MT1-MMP. NSC 405020 (100 μM) inhibits migration of cells with high level of MT1-MMP, with a reduction of migration efficiency by about 75%. NSC 405020 does not inhibit migration of cells with low level of MT1-MMP and does not inhibit cell adhesion on collagen.
• Cell Research:
  Assays are conducted in wells of a 96-well flat bottom, white wall plates. 184B5-MT and MCF7-β3/MT cells (5×104) are grown for 16 hours in MEGM-10% FBS and DMEM-10% FBS, respectively. 184B5-MT cells are replenished with fresh MEGM (0.1 mL per well) and incubated for an additional 24 hours in the presence of the compounds (100 μM) or vehicle (1% DMSO). MCF7-β3/MT cells are replenished with fresh DMEM-10% FBS (0.1 mL per well) and incubated for an additional 6 hours in the presence of the compounds (400 μM) or vehicle (2% DMSO). The viable cells are counted using a luminescent ATP-Lite assay.(Only for Reference)

Storage & Handling

• Storage: RT
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

NSC 405020, NSC-405020, NSC405020, MMP, NICD3, inhibit, Notch3, MT1-MMP, Matrix metalloproteinases, Inhibitor, hemostasis, vestibular schwannoma, thrombosis, WM852 cells

Compound Identifiers

PubChem CID

346721