NS-398

SKU: orb1304417

Description

NS-398 is a selective cyclooxygenase-2 (COX-2) inhibitor exhibiting anti-inflammatory properties. It is widely used in research to investigate COX-2's role in inflammation, cancer, and pain, utilizing both in vitro assays and in vivo animal models.

Research Area

Immunology & Inflammation, Neuroscience

Key Properties

• CAS Number: 123653-11-2
• MW: 314.36
• Purity: 99.00%
• Formula: C₁₃H₁₈N₂O₅S
• SMILES: CS(=O)(=O)Nc1cc(ccc1OC1CCCCC1)[N+]([O-])=O
• Target: COX
• Solubility: 10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (6.36 mM);DMSO:50 mg/mL (159.05 mM)

Bioactivity

• Target IC50:
  COX-2:3.8 μM
• In Vivo:
  NS398 could inhibit Cox-2 expression induced by acoustic injury and could attenuate noise-induced hearing threshold shifts and cochlear hair cell loss. The inhibition of Cox-2 by NS398 could attenuate Noise-induced hearing loss(NIHL)and related hair cell damage..
• In Vitro:
  NS-398 inhibits COX-2 enzyme activity in a concentration dependent manner, the IC50 being 3.8 μM, whereas NS-398 at 100 μM has no effect on COX-1 activity. At 10 μM, NS-398 treatment results in increased production of COX-2 and the pro-inflammatory cytokine. NS-398 (10 μM) induces apoptosis in LNCaP cells, but not in the more aggressive, androgen-unresponsive C4-2b cells. The C4-2b cells are observed to continue to proliferate when treated with NS-398 and continues to retain malignant phenotype characteristics. NS-398 treatment results in C4-2b cell differentiation into an unusual neuroendocrinelike cell. These neuroendocrine-like cells produces both epithelial (cytokeratin 18 and prostate specific antigen) and neuronal (neuron-specific enolase and chromogranin A) proteins. Furthermore, this C4-2b cellular response to NS-398 is mediated by NF-kB transcription factor activation. NS-398 induces NF-kB and down-regulates Ikβ-α protein expression in LNCaP C4-2b cells.
• Cell Research:
  1×106 cells are plated in six well cluster plates with 2 ml of medium for 24 h. At time point 0, medium was removed, cells were carefully washed with phosphate buffered saline (PBS) and serum free(SF), phenol-red free medium containing 0.5 μg/ml BSA was added. Incubations are continued for an additional 72 h with or without increasing NS-398 concentrations. NS-398 stocks (5 mM) are dissolved in 0.1% dimethylsulfoxide (DMSO). Cells and culture medium are harvested at 24 h time intervals. Dead cells are removed by gentle washing with PBS and cell number determined by direct counting using trypan blue dye exclusion to identify viable cells. DMSO (0.1%) is added to control cultures.(Only for Reference)

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

NS398, NS-398, NS 398, Inhibitor, inhibit, N-(2-cyclohexyloxy-4-nitrophenyl)methane sulfonamide, Cyclooxygenase, COX, COX-2

Compound Identifiers

PubChem CID

22406120