NS 1619

SKU: orb1226133

Description

NS1619 have been reported to have cardio-protective effects after ischemic-reperfusion injury.

Key Properties

• CAS Number: 153587-01-0
• MW: 362.23
• Purity: >98% (HPLC)
• Formula: C₁₅H₈F₆N₂O₂
• SMILES: O=C1NC2=CC(C(F)(F)F)=CC=C2N1C3=CC(C(F)(F)F)=CC=C3O
• Target: Potassium Channel
• Solubility: DMSO: > 10 mM

Bioactivity

• In Vivo:
  Opening of KCa channels with NS-1619 (1 mg/kg; i.p.) can delay protection in mouse hearts. Animal model: Adult male outbred ICR mice. Dosage: 1 mg/kg. Administration: Pretreated i.p. 24 h before I/R. Result: Pretreatment induced delayed protection 24 h later. Resulted in significant cardioprotection 24 h later, i. e. , infarct size was reduced from 38.8 ± 3.7% to 19.8 ± 2.9%.
• In Vitro:
  NS1619 (5, 10, 30, 50, and 100 μM) inhibits the proliferation of A2780 cells in a dosage and time dependent manner IC50 = 31.1 μM for 48 h pretreatment. NS1619 (30 μM) exhibits augmenting effect on whole cell IK in human ovarian cancer cells A2780.NS1619 (10, 30, 50, and 100 μM) increases levels of p53, p21Cip1 and Bax proteins in A2780 cells. DNA content of A2780 cells was significantly decreased after 36 and 48 h of pretreatment. The breakdown of DNA results in death of the tumor cells. Cell Viability Assay Cell line: The human ovarian cancer cell line A2780. Concentration: 5, 10, 30, 50, and 100 μM. Incubation time: 48 hours. Result: Inhibited cell growth in a time and concentration-dependent manner, IC50 = 31.1 μM. Proliferation was significantly inhibited at concentrations of NS1619 higher than 10 μM. Western blot analysis. Cell line: A2780 cells. Concentration: 0, 5, 10, 30, 50, and 100 μM. Incubation time: 48 hours. Result: Expression of p53, p21, and Bax in A2780 cells was significantly increased in comparison with control. Western blot analysis. Cell line: A2780 cells. Concentration: 30 μM. Incubation time: 36 and 48 hours. Result: DNA content of A2780 cells was significantly decreased after 36 and 48 h of pretreatment. The breakdown of DNA results in death of the tumor cells.

Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

NS1619 | NS-1619 | NS 1619.

Quick Database Links

Gene Symbol

Potassium Channel