Novobiocin Sodium

SKU: orb1310113

Description

Novobiocin Sodium is a coumarin-class antibiotic from Streptomyces niveus that inhibits bacterial DNA gyrase by blocking its ATPase activity. It is widely used in biochemical research to study DNA replication and bacterial resistance mechanisms in both in vitro and in vivo experimental models.

Research Area

Cell Biology, Infectious Disease & Virology, Metabolism Research, Molecular Biology, Pharmacology & Drug Discovery, Signal Transduction

Key Properties

• CAS Number: 1476-53-5
• MW: 634.61
• Purity: 99.00% (May vary between batches)
• Formula: C₃₁H₃₅N₂NaO₁₁
• SMILES: [Na+].COC1C(OC(N)=O)C(O)C(Oc2ccc3c(O)c(NC(=O)c4ccc([O-])c(CC=C(C)C)c4)c(=O)oc3c2C)OC1(C)C
• Target: Virus Protease,DNA gyrase,Antibiotic,Antibacterial,Apoptosis,Potassium Channel,Autophagy,DNA/RNA Synthesis,HSP,ABC Transporter,Topoisomerase
• Solubility: DMSO:83 mg/mL (130.79 mM)

Bioactivity

• Target IC50:
  HSP90:700 μM (IC50)
• In Vitro:
  Novobiocin also interacts with Hsp90, altering the affinity of the chaperone for geldanamycin and radicicol and causing in vitro and in vivo depletion of key regulatory Hsp90-dependent kinases including v-Src, Raf-1, and p185(ErbB2). Novobiocin interferes with association of the co-chaperones Hsc70 and p23 with Hsp90. Novobiocin specifically inhibits the maturation of the heme-regulated eIF2alpha kinase (HRI) in a concentration-dependent manner. Novobiocin induces the dissociation of Hsp90 and Cdc37 from immature HRI, while the Hsp90 cochaperones p23, FKBP52, and protein phosphatase 5 remained associated with immature HRI. Novobiocin causes morphological and biochemical changes which lead to induction of cell death exhibiting characteristic features of metazoan apoptosis. Novobiocin, a HSP90 inhibitor, could decrease the expression of SMYD3 and dose dependently inhibit the proliferation and migration of MDA-MB-231 human breast cancer cells. Novobiocin can inhibit the migration of breast cancer cells and such event may involve the downregulation of SMYD3. Novobiocin, an aminocoumarin antibiotic, interferes with heat shock protein 90 and hypoxia inducible factor dependent gene expression and thus compromises cell survival. Novobiocin (500 礛) results in a significant increase of [Ca(2+)]i, decrease of forward scatter, increase of annexin-V-binding and enhances ceramide formation. Novobiocin stimulates eryptosis, an effect at least in part due to entry of extracellular Ca(2+) and formation of ceramide.

Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

DNAgyrase, DNA interstrand cross-links, DNA gyrase, ABCG2, Albamycinsodium, Autophagy, anti-orthopoxvirus, Antibiotic, Bile salt export pump, Bacterial, Cathomycin, Cathomycinsodium, Mutz-2, Novobiocin, Novobiocin Sodium, HL60, Potassium Channel, PotassiumChannel, pneumococcal, Topo I

Compound Identifiers

PubChem CID

54730021