Norethindrone

SKU: orb1308376

Description

Norethindrone is a synthetic progestin used in research for its potent ovulation inhibition and effects on uterine tissue. It is applied in studies of hormonal contraception, endometriosis, and menstrual disorders, utilizing both in vitro receptor binding assays and in vivo animal models.

Research Area

Infectious Disease & Virology, Metabolism Research

Key Properties

• CAS Number: 68-22-4
• MW: 298.42
• Purity: 99.82%
• Formula: C₂₀H₂₆O₂
• SMILES: C[C@]12CC[C@H]3[C@@H](CCC4=CC(=O)CC[C@H]34)[C@@H]1CC[C@@]2(O)C#C
• Target: Progesterone Receptor,Antibacterial,Estrogen/progestogen Receptor
• Solubility: DMSO:20 mg/mL (67.02 mM);Ethanol:5 mg/mL (16.75 mM)

Bioactivity

• In Vitro:
  Norethisterone, or, is a 19-nortestosterone derivative, that lacks a C19 methyl group and possesses C17 ethinyl substitution, and primarily displays progestational activity rather than androgenic activity and, to a lesser extent, has oestrogenic and anti-oestrogenic activity. NET shows five- to eight-fold weaker progesterone receptor binding and transactivation activities than the Org 2058 (100%) and two-fold stronger than progesterone. Binding and transactivation activities of NET for androgen receptor (5α-dihydrotestosterone 100%) are 3.2 and 1.1%, respectively, for estrogen receptor none (estradiol 100%) and for glucocorticoid receptor below 1% (dexamethasone 100%). Norethisterone (1 nM) inhibits serum-stimulated or oestradiol (0.1 nM)-induced proliferation of MCF-7 by 41% and 34%, respectively. Norethisterone (50 nM) induces signi cant effects on rat osteoblast proliferation, differentiation, and mineralization processes, mimicking the effects of estradiol, which is mediated by estrogen receptor.
• Cell Research:
  96 well plates are seeded with approximately 1000 MCF-7 cells per well in assay kit medium. Subsequently, the cells are incubated with medium containing charcoal/dextran treated serum for three days. The Norethisterone is then added alone to the wells and incubated for seven days. To mimic continuous combined HRT the cells are treated with an oestradiol (0.1 nM)/ Norethisterone combination for seven days. After incubation for seven days, cell proliferation is measured by using an ATP-chemosensitivity test. In brief, proliferation is quantified by measuring light emitted during the bioluminescence reaction of luciferine in the presence of ATP and luciferase.(Only for Reference)

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

progestogen Receptor, progestogenReceptor, ProgesteroneReceptor, Progesterone Receptor, Inhibitor, ER, EstrogenReceptor, Estrogen Receptor, inhibit, NR3C3, Norethindrone, Norethisterone

Compound Identifiers

PubChem CID

6230