(±)-Norcantharidin

SKU: orb1301613

Description

(±)-Norcantharidin, also known as Endothall anhydride, is a synthetic small molecule with anticancer properties. It functions as a dual inhibitor targeting both c-Met and EGFR tyrosine kinases. Research applications include in vitro studies in human colon cancer cell lines and in vivo xenograft models investigating its antitumor mechanisms.

Research Area

Cardiovascular Research, Signal Transduction

Key Properties

• CAS Number: 29745-04-8
• MW: 168.15
• Purity: 99.43% (May vary between batches)
• Formula: C₈H₈O₄
• SMILES: O=C1OC(=O)C2C3CCC(O3)C12
• Target: EGFR,c-Met/HGFR
• Solubility: DMSO:60 mg/mL (356.82 mM)

Bioactivity

• In Vivo:
  NCTD might be a useful and inexpensive drug candidate to substitute for gefitinib to serve the treatment needs of cancer patients.NCTD inhibits the growth of xenografted tumors of human gallbladder carcinoma in nude mice by inducing apoptosis and blocking the cell cycle in vivo.
• Cell Research:
  Two human colon cancer cell lines, HCT116 and HT29, were used as model systems to investigate the anti-cancer molecular mechanism of NCTD. Cell cycle arrest and early/late apoptosis were analyzed by flow cytometry. The levels of EGFR, phospho-EGFR, c-Met, phospho-c-Met and other related proteins were quantified by western blot analysis
• Animal Research:
  The tumor xenograft model of human gallbladder carcinoma in nude mice in vivo was established with subcutaneous GBC-SD cells. The experimental mice were randomly divided into control, 5-FU, NCTD, and NCTD+5-FU groups which were given different treatments. Tumor growth in terms of size, growth curve, and inhibitory rate was evaluated. Cell cycle, apoptosis, and morphological changes of the xenografted tumors were assessed by flow cytometry and light/electron microscopy. The expression of the cell cycle-related proteins cyclin-D1 and p27 as well as the apoptosis-related proteins Bcl-2, Bax, and survivin were determined by the streptavidin-biotin complex (SABC) method and RT-PCR.

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

HGFR, EGFR, Endothall anhydride, Norcantharidin, (±) Norcantharidin, (±)Norcantharidin, cMet, c-Met, cMet/HGFR

Compound Identifiers

PubChem CID

93004