NO-prednisolone

SKU: orb1706115

Description

NCX-1015 is a nitric oxide-releasing derivative of prednisolone designed for research. It has been shown to effectively induce IL-10 production in vivo, making it a useful tool for studying immunomodulation and inflammation in experimental models.

Research Area

Immunology & Inflammation

Key Properties

• CAS Number: 327610-87-7
• MW: 539.57
• Purity: 99.31%
• Formula: C₂₉H₃₃NO₉
• SMILES: [H][C@@]12CC[C@](O)(C(=O)COC(=O)c3ccc(CO[N+]([O-])=O)cc3)[C@@]1(C)C[C@H](O)[C@@]1([H])[C@@]2([H])CCC2=CC(=O)C=C[C@]12C
• Target: Interleukin,IL Receptor
• Solubility: DMSO:40 mg/mL (74.13 mM)

Bioactivity

• In Vivo:
  In vivo, NO-prednisolone (NCX-1015) potently stimulates IL-10 production, suggesting that the NO steroid induces a regulatory subset of T cells that negatively modulates intestinal inflammation. The two doses of NO-prednisolone tested, 0.5 and 5 mg/kg/day effectively attenuate the severity of the wasting syndrome, ameliorate the colitis score, and reduce the colonic myeloperoxidase (MPO) activity. NO-prednisolone administration also reduces the colonic mRNA and protein content of tumor necrosis factor-α, IL-12, and IFN-γ. NO-prednisolone also reduces the expression of inducible NO synthase and cyclooxygenase-2. In a chronic model of granulomatous tissue inflammation, administration of NCX-1015 (13.9 μmol/kg）can effectively reduce the weight of dry granulomas, while exhibiting a lower anti-angiogenic effect.
• In Vitro:
  NO-prednisolone (NCX-1015) demonstrates superior effectiveness compared to Prednisolone in reducing inflammation, inhibiting the generation of cytokines and chemokines, and enhancing the expression of the steroid-sensitive cell-surface marker CD163 in human peripheral blood mononuclear cells. Additionally, NO-prednisolone exhibits greater potency than Prednisolone in inducing CD163 cell surface expression. This heightened efficacy of NO-prednisolone, relative to the parent molecule Prednisolone, is also observed in the inhibition of IL-1β production induced by LPS (lipopolysaccharide).'

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

NCX 1015, NCX1015, NCX-1015, NO-prednisolone, Interleukin Related, ILReceptor, IL-10, IL Receptor

Compound Identifiers

PubChem CID

9850209