NNMTi

SKU: orb1298655

Description

NNMTi (5-Amino-1-methylquinolinium) is a potent and selective inhibitor of nicotinamide N-methyltransferase (NNMT), with an IC50 of 1.2 µM. It effectively reduces cellular lipogenesis and 1-methylnicotinamide levels in adipocytes, serving as a key research tool for studying NNMT's role in metabolic disorders such as obesity and type 2 diabetes in vitro.

Key Properties

• CAS Number: 42464-96-0
• MW: 286.11
• Purity: 99.93% (May vary between batches)
• Formula: C₁₀H₁₁IN₂
• SMILES: [I-].C[n+]1cccc2c(N)cccc12
• Target: Others
• Solubility: 10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (6.99 mM);DMSO:33.3 mg/mL (116.39 mM);H2O:33.3 mg/mL (116.39 mM)

Bioactivity

• In Vivo:
  NNMTi treatment will rescue age-related deficits in muSC activity to promote superior regeneration post-injury in aging muscle. 24-month old mice were treated with saline (control), and low and high dose NNMTi (5 and 10 mg/kg) for 1-week post-injury, or control and high dose NNMTi for 3-weeks post-injury. All mice underwent an acute muscle injury (barium chloride injection) locally to the tibialis anterior (TA) muscle, and received 5-ethynyl-2'-deoxyuridine systemically to analyze muSC activity. In vivo contractile function measurements were conducted on the injured TA muscle and tissues collected for ex-vivo analyses, including myofiber cross-sectional area (CSA) measurements to assess muscle recovery. Results revealed that muscle stem cell proliferation and subsequent fusion were elevated in NNMTi-treated mice, supporting nearly 2-fold greater CSA and shifts in fiber size distribution to greater proportions of larger sized myofibers and fewer smaller sized fibers in NNMTi-treated mice compared to controls. Prolonged NNMTi treatment post-injury further augmented myofiber regeneration evinced by increasingly larger fiber CSA. Importantly, improved muSC activity translated not only to larger myofibers after injury but also to greater contractile function, with the peak torque of the TA increased by 70% in NNMTi-treated mice compared to controls. Similar results were recapitulated in vitro with C2C12 myoblasts, where NNMTi treatment promoted and enhanced myoblast differentiation with supporting changes in the cellular NAD+/NADH redox states.
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Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

regenerative, 5-Amino-1-methylquinolinium, Inhibitor, fusion, inhibit, muSC, muscle stem cell, NNMT, NNMTi

Compound Identifiers

PubChem CID

66522933