NNK

SKU: orb1217775

Description

NNK is a procarcinogen and a major tobacco-specific toxicant. It inhibits the expression of lysyl oxidase which is a tumor suppressor.

Key Properties

• CAS Number: 64091-91-4
• MW: 207.23
• Purity: >98% (HPLC)
• Formula: C₁₀H₁₃N₃O₂
• SMILES: CN(CCCC(c1cnccc1)=O)N=O
• Target: Lipoxygenase
• Solubility: DMSO:Soluble

Bioactivity

• In Vitro:
  NNK (100 pM; 0-60 min) stimulates activation of PKCα and MAPKs ERK1/2 that directly induce c-Myc phosphorylation. NNK (100 pM; 96 hours) enhances proliferation of cells expressing WT but not AA c-Myc mutant. Western blot analysis. Cell line: NCI-H82 cells. Concentration: 100 pM. Incubation time: 0-60 min. Result: Stimulated activation of PKCα and MAPKs ERK1/2 that directly induced c-Myc phosphorylation. Apoptosis Analysis Cell line: H1299 lung cancer cells. Concentration: 100 pM. Incubation time: 96 hours. Result: Enhanced proliferation of cells expressing WT but not T58A/S62A c-Myc mutant.

Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Nicotine-derived nitrosamine ketone

Quick Database Links

Gene Symbol

Lipoxygenase