NMS-873

SKU: orb1307322

Description

NMS-873 is a selective, allosteric inhibitor of the VCP/p97 ATPase. It is a valuable research tool used in vitro and in vivo to study protein homeostasis, ER-associated degradation (ERAD), and its role in cancer and neurodegenerative diseases.

Research Area

Neuroscience, Protein Biochemistry

Key Properties

• CAS Number: 1418013-75-8
• MW: 520.67
• Purity: 99.05% (May vary between batches)
• Formula: C₂₇H₂₈N₄O₃S₂
• SMILES: Cc1cc(OCc2nnc(SC3CCCC3)n2-c2cccnc2)ccc1-c1ccc(cc1)S(C)(=O)=O
• Target: p97
• Solubility: 10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (3.84 mM);DMSO:245 mg/mL (470.55 mM)

Bioactivity

• Target IC50:
  p97:30 nM
• In Vivo:
  NMS-873 activates the unfolded protein response, disrupts autophagy, and induces cell death. Across various blood and solid tumor cell lines, it inhibits the sensitivity of p97 to protease digestion, preventing the degradation of the linker-D2 domain, thereby exhibiting antiproliferative activity.
• Cell Research:
  Cells are seeded at 1,600 cells per well in 384-well white clear-bottom plates. Twenty-four hours after seeding, cells are treated with the compounds (eight dilution points, in duplicate, for each compound) and incubated for an additional 72 h at 37 °C under a 5% CO2 atmosphere. Cells are then lysed, and the ATP content in each well is determined using a thermostable firefly luciferase–based assay as a measure of cell viability. IC50 values are calculated using the percentage of growth of treated cells versus the untreated control. (Only for Reference)

Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

VCP, Cdc48, Inhibitor, inhibit, p97, NMS 873, NMS873, NMS-873

Compound Identifiers

PubChem CID

71521142