NLS-Cas12a Nuclease Protein

SKU: orb1496279

Description

Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-associated endonuclease from Lachnospiraceae bacterium，Cas12a, also known as Cpf1, is a 1200-1500 amino-acids long monomeric protein that belongs to the CRISPR/Cas system , an adaptive immune system of prokaryotes that has now become a powerful tool for genome editing. CRISPR/Cas12a belongs the class II (type 5) of the CRISPR/Cas system that is defined by a single-subunit effector. Cas12a has recently emerged as an alternative for Cas9, due to its distinct features such as the ability to target T-rich motifs, no need for trans-activating crR, inducing a staggered double-strand break and potential for both R processing and D nuclease activity. In addition, Cas12a is able to process more structured pre-CRISPR/R(crR) molecules into mature crRs which allows the possibility to use both mature or pre-crR for genome editing purposes. All these features make the CRISPR/Cas12a system a valuable genome-engineering tool. CRISPR-Cas12a has been successfully used to edit genomes in many mammalians cells and plants.

Research Area

Metabolism Research

Key Properties

• Source: NLS-Cas12a Nuclease (orb1496279) is expressed from E. coli cells. It contains AA Ser 2 - His 1228 (Accession # A0A5S8WF58).
• Expression System: E. coli
• Target: Cas12a
• Reactivity: Bacteria
• Tag: N-10×His
• Conjugation: Unconjugated

Storage & Handling

• Storage: -20°C
• Form/Appearance: Liquid
• Expiration Date: 6 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

CRISPR-associated endonuclease Cas12a, CRISPR-associated endonuclease Cpf1, cas12a, Cpf1

Quick Database Links

Gene Symbol

Cas12a