Nivolumab ELISA Kit

SKU: orb2975704

Description

PRINCIPLE OF THE ASSAY This assay employs the quantitative competitive enzyme immunoassay technique. Recombinant human PD1 antigen has been pre-coated onto a microplate. Standards or samples are premixed with biotin-labeled antibody and then pipetted into the wells. Nivolumab in the sample competitively binds to the pre-coated protein with biotin-labeled Nivolumab. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in inversely proportion to the amount of Nivolumab bound in the initial step. The color development is stopped and the intensity of the color is measured.

Key Properties

• Sample Types: Plasma, Serum
• Assay Type: Quantitative
• Range: 0.31-5 μg/mL
• Sensitivity: 0.156 μg/ml

Storage & Handling

• Storage: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 20% prior to the expiration date under appropriate storage condition.
• Disclaimer: For research use only

Alternative Names

BMS-936558, MDX-1106, ONO-4538, CAS: 946414-94-4