NH125

SKU: orb1300621

Description

NH125

Research Area

Cancer Biology

Key Properties

• CAS Number: 278603-08-0
• MW: 524.56
• Purity: >98%
• Formula: C₂₇H₄₅IN₂
• SMILES: [I-].CCCCCCCCCCCCCCCCn1cc[n+](Cc2ccccc2)c1C
• Target: Kinase
• Solubility: Soluble in DMSO (up to at least 25 mg/ml) or in Ethanol (up to at least 25 mg/ml)

Bioactivity

• Target IC50:
  eEF2K:60 nM
• In Vivo:
  NH125 reduces blood pressure in SHR and ROS production, induction of inflammatory molecules, and hypertrophy in SHR superior mesenteric artery.
• In Vitro:
  In C6 glioma cells, NH125 decreases the cellular content of phospho-eEF-2 without affecting total content eEF-2 content, and blocks cell cycle transit at the G1-S boundary. NH125 potently inhibits cell viability of 10 cancer cells with IC50 ranging from 0.7 to 4.8 μM. NH125 effectively inhibits histidine protein kinases, including Envz, PhoQ, BvgS, EvgS, and thus produces potent anti-bacteria activities on oxacillin-resistant Staphylococcus aureus (ORSA), vancomycin-resistant Enterococcus faecalis (VRE), penicillin-resistant Streptococcus pneumoniae (PRS), and other Gram-positive and Gram-negative bacteria. EEF2K inhibition by NH125 renders tumor cells more sensitive to curcumin and velcade, which possess ER stress-inducing action.
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• Cell Research:
  The viability of cells is measured using an MTT assay. Briefly, 5 × 104 cells are plated in 96-well plates and exposed to various concentrations of drug for 48–72 h. The formazan product formed after 4 h incubation with MTT is dissolved in 100% DMSO and read at 550 nM using a Dynatech Microplate Reader MR5000. (Only for Reference)
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Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Calmodulin-dependent protein kinases, Calmodulin-dependent kinases, CaMK, CaMKII, Autophagy, Bacterial, eEF-2 kinase, Fungal, Inhibitor, inhibit, NH 125, NH125, NH-125, Virus Protease, VirusProtease, PKA, PKC

Compound Identifiers

PubChem CID

10436839