NFkB p65 antibody

• Catalog Number: orb344390
• Category: Antibodies
• Description: NFkB p65 antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 27F9.G4
• Tested applications: ELISA, IF, IHC, WB
• Reactivity: Human
• Isotype: IgG2a
• Immunogen: NFkB p65 (Rel A) peptide corresponding to a region near the C-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
• Concentration: 0.98 mg/mL
• Dilution range: ELISA: 1:50,000-1:100,000, IHC: 1:200-1:600, IF: 1:5000, WB: 1:1000 - 1:5000
• Form/Appearance: Liquid (sterile filtered)
• Purity: This product was purified from concentrated tissue culture supernate by Protein A chromatography and showed a single band by IEP (immunoelectrophoresis) when tested with anti-mouse antibody. Reactivity was confirmed by ELISA against peptide conjugated carrier protein and by Western blot against HeLa whole cell lysate.
• Conjugation: Unconjugated
• UniProt ID: Q04206
• NCBI: 223468676
• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: mouse anti-NF-kB p65 Antibody, mouse anti-Rel A an
• Note: For research use only
• Application notes: This protein A purified mouse monoclonal antibody is directed against NFkB p65 (Rel A) and a 1:1000 dilution recognizes a 65 kD band by Western blot against HeLa whole cell lysate. Control peptide (100-4165p) is sold separately. This product tested in WB, ICC, IHC, and IF.
• Expiration Date: 12 months from date of receipt.

Immunofluorescence analysis of mouse endothelial cells using NFkB p65 antibody

Western blot analysis of used to detect p65 using NFkB p65 antibody

Immunofluorescence analysis of Human Fibroblasts tissue using NFkB p65 antibody

Immunofluorescence analysis of Hela cells using NFkB p65 antibody

Immunohistochemical staining of human tissues showed moderate to strong staining within many tissues, including epithelium of the breast, small intestine, kidney, pancreas, prostate, skin, placenta, and uterus, as well as within neurons and lymphoid tissu

Western blot analysis of HeLa whole cell lysate using NFkB p65 antibody