β-Neo-Endorphin

SKU: orb3053954

Description

β-Neo-Endorphin is an endogenous opioid peptide. β-Neo-Endorphin is a hypothalamic “big” Leu-enkephalin of porcine origin. β-Neo-Endorphin shows activation of the Erk1/2, MMP-2 and MMP-9.

Key Properties

• CAS Number: 77739-21-0
• MW: 1100.3
• Purity: >98% (HPLC)
• Formula: C₅₄H₇₇N₁₃O₁₂
• Solubility: H2O : 100 mg/mL (90.89 mM; ultraphonic)

Bioactivity

• In Vitro:
  β-Neo-Endorphin (0-20 μM, 20 h) stimulates wound healing in human keratinocytes. β-Neo-Endorphin (promotes wound healing through activation of the Erk1/2 signaling pathway in human keratinocytes. β-Neo-Endorphin (0-20 μM, 12 h) stimulates keratinocyte migration through activation of metalloproteinase (MMP)-2 and -9. β-Neo-Endorphin (12 h) stimulates migration in the fibroblast. Cell Viability Assay Cell line: Human keratinocytes (HaCaT). Concentration: 0, 5, 10 and 20 μM. Incubation time: 20 h. Result: Significantly increased the wound closure. Had no observable effect on cell viability. Western blot analysis. Cell line: Human keratinocytes (HaCaT). Concentration: 0, 5, 10 and 20 μM. Incubation time: 12 h. Result: Activated Erk1/2, phosphorylated P90RSK and Elk-1, showed significant upregulation of MMP-2 and -9 expression.

Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only