Necrostatin-34

SKU: orb1298374

Description

Necrostatin-34 is a potent and selective RIPK1 kinase inhibitor that stabilizes the kinase in an inactive state. It is widely used in research to investigate necroptosis in both cellular and animal models of inflammatory and degenerative diseases.

Research Area

Cell Biology, Signal Transduction

Key Properties

• CAS Number: 375835-43-1
• MW: 384.48
• Purity: 98.80% (May vary between batches)
• Formula: C₁₈H₁₆N₄O₂S₂
• SMILES: Cc1ccc(cc1)C1CC(=O)NC(SCC(=O)Nc2nccs2)=C1C#N
• Target: RIP kinase
• Solubility: 10% DMSO+40% PEG300+5% Tween 80+45% Saline:4 mg/mL (10.4 mM);DMSO:125 mg/mL (325.11 mM)

Bioactivity

• In Vitro:
  Necrostatin-34 (10 μM) inhibits the dimerization-induced RIPK1 activation as examined by phosphorylation of Ser166 (p-S166) of RIPK1, a biomarker for RIPK1 activation. Necrostatin-34 may block TNFα-induced complex II formation by inhibiting the activation of RIPK1 kinase. Necrostatin-34 shows IC50 values of 667 nM and 134 nM for TNFα in FADD def-Jurkat cells and L939 cells, respectively.

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

2-{[3-cyano-4-(4-methylphenyl)-6-oxo-1,4, Necroptosis, Necrostatin 34, Necrostatin34, Necrostatin-34, inhibit, Inhibitor, FADD, RIPK, RIPK1, RIPkinase, RIP kinase, p-S166, Receptor-interacting protein kinases

Compound Identifiers

PubChem CID

3841655