NCI 126224

SKU: orb1979970

Description

NCI 126224, a toll-like receptor 4 (TLR4) antagonist, demonstrates selective inhibition of nitric oxide (NO) production in RAW 264.7 macrophages, triggered by the TLR4 agonist LPS (IC50 = 0.31 µM), as opposed to minimal effects on NO production induced by agonists for TLR7/8 (R-848), TLR1/2 (Pam3CSK4), and TLR3 (poly(I:C)). Nevertheless, it also effectively inhibits NO production initiated by the TLR2/6 agonist FSL-1 at an IC50 of 0.6 µM in the same cell line. Moreover, NCI 126224 suppresses LPS-induced NF-κB activity in BV-2 microglial cells and reduces LPS-triggered increases in IL-1β and TNF-α levels in RAW 264.7 macrophages with IC50 values of 5.92, 0.42, and 1.54 µM, respectively.

Key Properties

• CAS Number: 65974-52-9
• MW: 265.20
• Formula: C₁₂H₁₁NO₆
• SMILES: COC(/C(C(OC)=O)=C/C1=C(C=CC=C1)[N+]([O-])=O)=O
• Target: TLR

Storage & Handling

• Storage: -20°C
• Disclaimer: For research use only

Quick Database Links

Gene Symbol

TLR