N1-Methylpseudouridine

SKU: orb1940604

Description

N1-Methylpseudouridine is a methylpseudouridine and enhances translation through eIF2α-dependent and independent mechanisms by increasing ribosome density in mRNA.(In Vitro):In HEK293T cells, Incorporation of N1-Methylpseudouridine into mRNA modifies mRNAs produced higher amounts of luc than the standard Luc mRNA . Incorporation of N1-Methylpseudouridine nucleoside modification in both Luc and GFP mRNA enhances the initiation step of translation, in part by suppressing eIF2α phosphorylation. In addition, polysome formation and growth on the N1-Methylpseudouridine-containing Luc mRNA is enhanced due to the reduction of elongation rate.(In Vivo):mRNAs containing the N1-Methylpseudouridine modification alone and/or in combination with 5-methylcytidine (m5C) outperformed the current state-of-the-art pseudouridine (Ψ) and/or m5C/Ψ-modified mRNA platform by providing up to ~44-fold (when comparing double modified mRNAs) or ~13-fold (when comparing single modified mRNAs) higher reporter gene expression upon transfection into cell lines or mice, respectively. We show that (m5C/)N1-Methylpseudouridine-modified mRNA resulted in reduced intracellular innate immunogenicity and improved cellular viability compared to (m5C/)Ψ-modified mRNA upon in vitro transfection.

Key Properties

• CAS Number: 13860-38-3
• MW: 258.23
• Purity: >98% (HPLC)
• Formula: C₁₀H₁₄N₂O₆
• SMILES: Cn1cc([C@@H]2O[C@H](CO)[C@@H](O)[C@H]2O)c(=O)[nH]c1=O
• Target: Antibacterial
• Solubility: In Vitro: DMSO : 125 mg/mL (484.06 mM)

Bioactivity

• In Vivo:
  Animal model: 7-week-old Balb/c mice. Dosage: 20 μg. Administration: I. m. or i. d. routes for 21 days. Result: had a higher translational capacity.

Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

1-Methylpseudouridine

Quick Database Links

Gene Symbol

Antibacterial