N-tert-butyl-α-Phenylnitrone

SKU: orb1305741

Description

N-tert-butyl-α-phenylnitrone (PBN) is a well-characterized spin trap and cyclooxygenase-2 (COX-2) inhibitor. Its ability to scavenge reactive oxygen species and cross the blood-brain barrier supports its use in neuroprotection, anti-inflammatory, and metabolic disease research in both cellular and animal models.

Research Area

Immunology & Inflammation, Metabolism Research, Neuroscience, Signal Transduction

Key Properties

• CAS Number: 3376-24-7
• MW: 177.24
• Purity: 99.84% (May vary between batches)
• Formula: C₁₁H₁₅NO
• SMILES: CC(C)(C)[N+]([O-])=CC1=CC=CC=C1
• Target: COX,ROS,Reactive Oxygen Species
• Solubility: DMSO:50 mg/mL (282.1 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (11.28 mM)

Bioactivity

• In Vivo:
  In C57Bl/6 mice induced by lipopolysaccharide (LPS), intraperitoneal injection of 100 mg/kg N-tert-butyl-α-Phenylnitrone twice a day (on gestational day 8) abolishes LPS-induced lipid peroxidation, nitrate tyrosine residue levels and GSH depletion, and reduces the incidence of external malformations.
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• In Vitro:
  Treatment of 25-100 μM N-tert-butyl-α-Phenylnitrone significantly reduces 2,2'-azobis (2-amidinopropane) dihydrochloride (AAPH)-induced intracellular ROS accumulation. N-tert-butyl-α-Phenylnitrone also attenuates AAPH-induced cytotoxicity, matrix degradation, and apoptosis, inhibiting AAPH-induced ERK/MAPK pathway activation.
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Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Cyclooxygenase, COX-2, COX, (Z)-N-benzylidene-2-Methylpropan-2-aMine oxide, adducts, BBB, antioxidant, ERK, Inhibitor, iNOS, MAPK, inhibit, N tert butyl α Phenylnitrone, neuroprotective, N-benzylidene-2-Methylpropan-2-aMine Oxide, N-tert-butyl-alpha-Phenylnitrone, N-tert-butyl-a-Phenylnitrone, NtertbutylαPhenylnitrone, N-tert-butyl-α-Phenylnitrone, nitroxide, Reactive Oxygen Species, ROS, Scavenger, spin

Compound Identifiers

PubChem CID

4390