N-(p-amylcinnamoyl) Anthranilic Acid

SKU: orb1301778

Description

ACA is a dual-action small molecule inhibitor, functioning as a broad-spectrum phospholipase A2 (PLA2) antagonist and a transient receptor potential (TRP) channel blocker. It is utilized in research to investigate inflammatory pathways and calcium signaling in both cellular and animal models.

Research Area

Metabolism Research, Pharmacology & Drug Discovery

Key Properties

• CAS Number: 110683-10-8
• MW: 337.41
• Purity: 99.75% (May vary between batches)
• Formula: C₂₁H₂₃NO₃
• SMILES: O=C(C1=C(NC(C=CC2=CC=C(CCCCC)C=C2)=O)C=CC=C1)O
• Target: Phospholipase,TRP/TRPV Channel
• Solubility: DMSO:125 mg/mL (370.47 mM);10% DMSO+40% PEG300+5% Tween-80+45% Saline:3.3 mg/mL (9.78 mM);H2O:Insoluble

Bioactivity

• In Vitro:
  N-(p-amylcinnamoyl) Anthranilic Acid modulates different TRP channels most probably by a direct interaction. Owing to its high potency and efficacy on TRPM2, N-(p-amylcinnamoyl) Anthranilic Acid can serve, in combination with other blockers, as a pharmacological tool for studying H2O2-induced Ca2+ signalling and biological functions of TRPM2 channels in native cells.N-(p-amylcinnamoyl) Anthranilic Acid is also an inhibitor of phospholipase A2, blocking the release of arachidonic acid when given at 50 μM.
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• Cell Research:
  Measurements of [Ca2+]i were carried out on TRPM8-transfected HEK293 cells grown in 96-well microplates. Cells were loaded with 1 μm fluo-4 for 30 min at 37°C in a standard solution. Subsequently, the standard solution was exchanged for a solution containing 1 mm EGTA instead of 2 mm CaCl2, and plates were inserted into a fluorometric-imaging plate reader. The fluorescence was excited at 485 nm, emitted at 535 nm and corrected for the background fluorescence. Fluorescence values were measured after addition of ACA, menthol and 3 mm CaCl2 to the EGTA-containing solution. ACA, menthol and 3 mm CaCl2 were applied consecutively in the given order with a time delay of 2 min between each application.
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Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

N(pamylcinnamoyl) Anthranilic Acid, N-(p-amylcinnamoyl) Anthranilic Acid, inhibit, N (p amylcinnamoyl) Anthranilic Acid, Inhibitor, ACA, PLA2, Phospholipase, Transient receptor potential channels, TRP Channel, TRPVChannel, TRPV Channel, TRPChannel

Compound Identifiers

PubChem CID

1974