N-Ethylmaleimide

SKU: orb1304555

Description

N-Ethylmaleimide (NEM) is a small molecule alkylating agent that covalently modifies cysteine residues. It is widely used in biochemical research as an inhibitor of cysteine proteases, deubiquitinating enzymes, and mitochondrial phosphate transport in both in vitro and cell-based studies.

Research Area

Protein Biochemistry

Key Properties

• CAS Number: 128-53-0
• MW: 125.13
• Purity: >98%
• Formula: C₆H₇NO₂
• SMILES: C(C)N1C(=O)C=CC1=O
• Target: Protease
• Solubility: DMSO:237.5 mg/mL (1898.03 mM);5% DMSO+95% Saline:1.2 mg/mL (9.59 mM);H2O:50 mg/mL (399.58 mM)

Bioactivity

• Target IC50:
  KB-V1 cells:0.016 mM|prolyl endopeptidase:6.3 μM|NCI-H4 cells:6.7 μg/mL (GL50)|COS-7 cells:43.7 μM (EC50)|SF-268 cells:6.5 μg/mL (GL50)|KB-3-1 cells:0.03 mM|MCF-7 cells:9.9 μg/mL (GL50)
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• In Vivo:
  METHODS: To examine the potential enhancement of sleep properties induced by alprazolam (Alp), N-Ethylmaleimide (1 mg/kg, 1% CMC-Na) and Alp (1.84 mg/kg, 1% CMC-Na) were administered by gavage to C57BL/6J mice. RESULTS: Combined administration of Alp and NEM significantly reduced sleep latency and prolonged sleep duration compared with Alp alone. This effect was characterized by a significant increase in REM duration.
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• In Vitro:
  METHODS: Growth-arrested VSMC cells were treated with N-Ethylmaleimide (20 µM) and PDGF-BB (20 ng/mL) for 5 min - 2 h, and the expression levels of target proteins were detected by Western Blot. RESULTS: N-Ethylmaleimide inhibited PDGF BB-stimulated Akt phosphorylation. METHODS: Jurkat T cells were treated with N-Ethylmaleimide (3-100 µM) for 1-20 min, and target protein expression levels were detected by immunoblots. RESULTS: Treatment of intact cells with 50-100 µM N-Ethylmaleimide for 5-10 min resulted in a significant increase in tyrosine residue phosphorylation.
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Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

1-Ethyl-1H-pyrrole-2,5-dione, Cathepsin, Apoptosis, DUBs, Deubiquitinase, Cysteine protease, cysteine residue, CysteineProtease, Cysteine Protease, NEM, NEthylmaleimide, N-Ethylmaleimide, modify, N Ethylmaleimide, inhibit, Inhibitor, Ethylmaleimide, sulfhydryl, phosphate transport, VSMC

Compound Identifiers

PubChem CID

4362