N-Ethylmaleimide

SKU: orb1226612

Description

N-ethylmaleimide is a sulfhydryl reagent that is widely used in experimental biochemical studies.(In Vitro):N-Ethylmaleimide (20 μM;30 min) inhibits Akt Ser-473, Akt Thr-308 , p70S6K, ribosomal protein S6, 4E-BP1, eIF4E, BAD and FKHR-L1 phosphorylation.N-Ethylmaleimide (20 μM;30 min) affects conversion of pro-caspase-3 in vascular smooth muscle cells.N-Ethylmaleimide (20 μM;6 h) promotes vascular smooth muscle cells apoptosis.N-Ethylmaleimide (20 μM;30 min) affects PP2A activity and ROS production in vascular smooth muscle cells.(In Vivo):N-Ethylmaleimide (10 mg/kg; i.h.) promotes the prevalence situation of mice with acute gastric ulcers.

Key Properties

• CAS Number: 128-53-0
• MW: 125.13
• Purity: >98% (HPLC)
• Formula: C₆H₇NO₂
• SMILES: CCN1C(=O)C=CC1=O
• Target: Cysteine Protease
• Solubility: DMSO: 10 mM

Bioactivity

• In Vivo:
  N-Ethylmaleimide (10 mg/kg; i.h.) promotes the prevalence situation of mice with acute gastric ulcers. Animal model: Male Wistar rats with acute gastric ulcers induced by absolute ethanol injection. Dosage: 10 mg/kg. Administration: Subcutaneous injection; 10 mg/kg once. Result: Increased the lesion area of acute gastric ulcers and attenuated the gastroprotective effect of PAG in rats.
• In Vitro:
  N-Ethylmaleimide (20 μM; 30 min) inhibits Akt Ser-473, Akt Thr-308, p70S6K, ribosomal protein S6, 4E-BP1, eIF4E, BAD and FKHR-L1 phosphorylation. N-Ethylmaleimide (20 μM; 30 min) affects conversion of pro-caspase-3 in vascular smooth muscle cells. N-Ethylmaleimide (20 μM; 6 h) promotes vascular smooth muscle cells apoptosis. N-Ethylmaleimide (20 μM; 30 min) affects PP2A activity and ROS production in vascular smooth muscle cells. Western blot analysis. Cell line: Vascular smooth muscle cells. Concentration: 20 μM. Incubation time: 2 hours. Result: Effectively inhibited platelet-derived growth factor-BB (PDGF-BB)-stimulated Akt Ser-473, Akt Thr-308, p70S6K, ribosomal protein S6, 4E-BP1, BAD and FKHR-L1 phosphorylation with a concentration of 20 μM. Western blot analysis. Cell line: Vascular smooth muscle cells. Concentration: 20 μM. Incubation time: 2 hours. Result: Increased of 1.8-fold in the conversion of pro-caspase-3 into active form, and showed better effect with 20 ng/ml PDGF-BB adding. Apoptosis Analysis Cell line: Vascular smooth muscle cells. Concentration: 20 μM. Incubation time: 6 hours. Result: Induced vascular smooth muscle cells apoptosis by 3-fold, and exhibited 5-fold apoptosis with 20 ng/ml PDGF-BB adding. Cell Viability Assay Cell line: Vascular smooth muscle cells. Concentration: 20 μM. Incubation time: 30 min. Result: Increased PP2A activity 1.7-flod and increased ROS production 2-fold in vascular smooth muscle cells.

Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Quick Database Links

Gene Symbol

Cysteine Protease