MUC5AC Antibody

• Catalog Number: orb749695
• Category: Antibodies
• Description: This mAb recognizes the peptide core of gastric mucin M1 (recently identified as Mucin 5AC). This mucin is present in primary ovarian mucinous cancer but usually absent in colorectal adenocarcinoma, thus showing an expression pattern opposite to MUC2. Together with a panel of antibodies, anti-MUC5AC may be useful for differential identification of primary mucinous ovarian tumors from colon adenocarcinoma metastatic to the ovary. MUC5AC antibodies may also be useful for identification of intestinal metaplasia as well as in the identification of pancreatic carcinoma and pre-cancerous changes vs. normal pancreas. It has been found that treating the testing sample with Papain or mercaptoethanol will destroy the M1 epitope for the 2-11M1 antibody (Ref. 1; Cancer Research 46.8 (1986): 3983-3989.)
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG1, kappa
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Immunogen: An M1 mucin preparation from the fluid of an ovarian mucinous cyst belonging to an O Le(a-b) patient was used as the immunogen for the MUC5AC antibody.
• UniProt ID: P98088
• Tested applications: ELISA, FACS, IF, WB
• Dilution range: ELISA: 2-4ug/ml for coating (order BSA/sodium azide-free format),Western blot: 1-2ug/ml,Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 1-2ug/ml
• Application notes: Optimal dilution of the MUC5AC antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Antibody Type: Primary Antibody
• Clone Number: 2-11M1
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Research Area: Epigenetics
• Note: For research use only

SDS-PAGE Analysis of Purified, BSA-Free MUC5AC Antibody (clone 2-11M1). Confirmation of Integrity and Purity of the Antibody.