MUC1 Antibody / Mucin-1

• Catalog Number: orb749682
• Category: Antibodies
• Description: This mAb reacts with MUC1/Mucin-1/Epithelial Marker Antigen/EMA. The dominant epitope of this mAb has not yet been determined. MUC1 is a large cell surface mucin glycoprotein expressed by most glandular and ductal epithelial cells and some hematopoietic cell lineages. It is expressed on most secretory epithelium, including mammary gland and some hematopoietic cells. It is expressed abundantly in lactating mammary glands and over expressed abundantly in > 90% breast carcinomas and metastases. Transgenic MUC1 has been shown to associate with all four c-erbB receptors and localize with c-erbB1 (EGFR) in lactating glands. The MUC1 gene contains seven exons and produces several different alternatively spliced variants. The major expressed form of MUC1 uses all seven exons and is a type 1 transmembrane protein with a large extracellular tandem repeat domain. The tandem repeat domain is highly O glycosylated and alterations in glycosylation have been shown in epithelial cancer cells. Antibody to EMA is useful as a pan-epithelial marker for detecting early metastatic loci of carcinoma in bone marrow or liver.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 139H2
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: Human milk-fat globule membranes (HMFGM) was used as the immunogen for the MUC1 antibody.
• Antibody Type: Primary Antibody
• Dilution range: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This MUC1 antibody is available for research use only.
• UniProt ID: P15941
• Storage: Store the MUC1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the MUC1 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC: Formalin-fixed, paraffin-embedded human lung cancer stained with MUC1 antibody (clone 139H2).

IHC: Formalin-fixed, paraffin-embedded human breast cancer stained with MUC1 antibody (clone 139H2).

IHC: Formalin-fixed, paraffin-embedded human endometrial carcinoma stained with MUC1 antibody (clone 139H2).

Western blot testing of human T47D cell lysate with MUC1 antibody. This glycoprotein is commonly visualized between 120~500 kDa.

Western blot testing of human MCF7 cell lysate with MUC1 antibody. This glycoprotein is commonly visualized between 120~500 kDa.