MTBP antibody

• Catalog Number: orb345266
• Category: Antibodies
• Description: MTBP antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: PHL-1
• Tested applications: ELISA, WB
• Reactivity: Human
• Isotype: ASCITES
• Immunogen: This monoclonal antibody was produced by repeated immunizations with a recombinant amino-terminal HIS6X-tagged polypeptide corresponding to amino acid residues 667-812 of human MTBP protein.
• Concentration: Neat
• Dilution range: ELISA: 1:2,000 - 1:20,000, WB: 1:200 - 1:2,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: This antibody is directed against human MTBP protein. The product was produced from clarified mouse ascetic fluid. A BLAST analysis was used to suggest reactivity with this protein from human (100% homology) and chimpanzee. Cross reactivity with MTBP protein from mouse (77% homology) and chicken (79% homology) is unlikely due to the relatively low number of positive residues and high number of gaps within this sequence. Cross reactivity with MTBP homologues from other sources has not been determined.
• Conjugation: Unconjugated
• UniProt ID: Q96DY7
• NCBI: NP_071328.2
• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: mouse anti-MTBP antibody, mouse anti-mouse double
• Note: For research use only
• Application notes: This antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band ~ 110 kDa in size corresponding to MTBP by western blotting in the appropriate cell lysate or extract.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of human 293 cell lysates with (+) and without (-) transfection with a full-length using MTBP antibody