MSA-2

SKU: orb1298624

Description

MSA-2 is a potent, orally bioavailable non-nucleotide STING agonist. Its non-covalent dimer binds STING with nanomolar affinity, inducing interferon-β secretion and demonstrating robust anti-tumor activity and immune memory in syngeneic mouse models, including synergy with anti-PD-1 therapy.

Research Area

Immunology & Inflammation

Key Properties

• CAS Number: 129425-81-6
• MW: 294.32
• Purity: 98.88% (May vary between batches)
• Formula: C₁₄H₁₄O₅S
• SMILES: COc1cc2cc(sc2cc1OC)C(=O)CCC(O)=O
• Target: STING
• Solubility: DMSO:255 mg/mL (866.4 mM);0.5% CMC-Na:12.5 mg/mL (42.47 mM)

Bioactivity

• Target IC50:
  STING isoforms HAQ (human):24 μM|STING isoforms WT (human):8.3 μM
• In Vivo:
  METHODS: The EMT-6 mouse model was treated with MSA-2 (50 mg/kg, orally), and the changes in intratumoral cytokines and chemokines after MSA-2 treatment in vivo were explored. RESULTS IFN-β, IL-6, TNF-α, IFN-γ, and classical activation-associated chemokines including CCL2, CCL3, CCL4, CCL5, CXCL1, CXCL9, and CXCL10 were significantly upregulated in EMT-6 tissues. METHODS: MSA-2 was orally administered at a single dose of 50 mg/kg to mice bearing U14 and TC-1 cervical tumor models. Mice were treated with 5 mg/kg of anti-PD-1 every other day for a total of 3 treatments. Tumor volume was assessed every other day. RESULTS The tumor volume of mice was significantly reduced.
• In Vitro:
  METHODS: PK-15 cells were treated with MSA-2 at concentrations of 20, 30, 40 and 50 μM for 24 hours and then infected with SVV at 10 MOI. SVV RNA levels in treated cells were detected by RT-qPCR at 24 h post-infection (hpi). RESULTS Viral RNA levels in MSA-2-treated cells were significantly reduced in a dose-dependent manner.
• Animal Research:
  Animal Model was MC38 tumor-bearing C57BL6 mice,and The dosage was 60 mg/kg.Administration was P.o.;s.c (50 mg/kg);single dose

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

MSA 2, MSA2, MSA-2, MPYS, immunity, inhibit, Inhibitor, MITA, ERIS, interferon-β, MC38, secretion, TMEM173, TNF-α, Stimulator of Interferon Genes, STING, tumor

Compound Identifiers

PubChem CID

23035251