MS-444

SKU: orb1706943

Description

MS-444 (BE-34776) is a dual inhibitor of smooth muscle myosin light chain kinase (MLCK) and the RNA-binding protein HuR. It exhibits antitumor activity in preclinical models, including triple-negative breast cancer and colorectal cancer, and is used in research to study post-transcriptional gene regulation and cancer cell proliferation in vitro.

Research Area

Cell Biology, Metabolism Research, Signal Transduction

Key Properties

• CAS Number: 150045-18-4
• MW: 230.22
• Purity: 99.45% (May vary between batches)
• Formula: C₁₃H₁₀O₄
• SMILES: O=C1C=2C(O)=CC=C(O)C2CC3=COC(=C13)C
• Target: Myosin,Serine/threonin kinase
• Solubility: DMSO:40 mg/mL (173.75 mM)

Bioactivity

• Target IC50:
  YAMC cells:28.16 μM|RKO cells:5.60 μM|SW480 cells:10.98 μM|HCA7 cells:12.84 μM|HT29 cells:14.21 μM|RIE-1 cells:40.70 μM|HCT116 cells:10.98 μM
• In Vivo:
  Mice bearing HCT116 cell xenografts receive intraperitoneal injections of MS-444 (25 mg/kg) or vehicle every 48 hr to test the effects of MS-444 on CRC cell growth in vivo. Anti-tumor effects of MS-444 are observed with an approximately 1.7-fold reduction in tumor size. Mice treated with MS-444 shows a marked 2- to 3-fold reduction in microvessel density (MVD), showing the anti-angiogenic potential of MS-444.
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• In Vitro:
  MS-444 is a small molecule RNA-binding protein HuR (ELAVL1) inhibitor. Colorectal cancer (CRC) cells that show HuR overexpression are treated with MS-444 (1-100 μM) for 48 hr (HCT116 IC50=10.98 μM, HCA-7 IC50=12.84 μM, RKO IC50=5.60 μM, HT-29 IC50=14.21 μM, and SW480 IC50=10.98 μM). Contrasting effects are observed using non-transformed small intestinal (RIE-1 IC50=40.70 μM) and colonic epithelial cells (YAMC IC50=28.16 μM).
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Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

BE34776, BE-34776, BE 34776, myosin, Myosin, MS 444, MS444, MS-444, MLCK, Serine kinase, threoninkinase, threonin kinase, Serinekinase

Compound Identifiers

PubChem CID

132904