MRT68921

SKU: orb1300364

Description

MRT68921 is a potent dual inhibitor of the autophagy-initiating kinases ULK1 and ULK2, exhibiting IC50 values of 2.9 nM and 1.1 nM, respectively. This compound is a valuable research tool for investigating autophagy mechanisms and has been utilized in both cellular and in vivo studies to probe autophagy-dependent processes in cancer and other diseases.

Research Area

Cell Biology

Key Properties

• CAS Number: 1190379-70-4
• MW: 434.58
• Purity: >99.99% (May vary between batches)
• Formula: C₂₅H₃₄N₆O
• SMILES: N(CCCNC(=O)C1CCC1)C=2C(=CN=C(NC=3C=C4C(=CC3)CN(C)CC4)N2)C5CC5
• Target: Autophagy
• Solubility: H2O:< 1 mg/mL (insoluble or slightly soluble);DMSO:10 mg/mL (23.01 mM);Ethanol:< 1 mg/mL (insoluble or slightly soluble);10% DMSO+40% PEG300+5% Tween 80+45% Saline:1 mg/mL (2.3 mM)

Bioactivity

• Target IC50:
  ULK1:2.9 nM|ULK2:1.1 nM
• In Vitro:
  MRT68921 specifically disrupts autophagosome maturation and thus blocks autophagic flux through ULK1 inhibition.
• Cell Research:
  MEFs and 293T cells are grown in DMEM, supplemented with 10% fetal bovine serum and penicillin/streptomycin, and cultured at 37°C, 5% CO2. For induction of autophagy, cells are typically grown to 75% confluency, ished twice, and incubated in Earle's balanced salt solution (EBSS) for 1 h (or complete medium as a control). MRT67307 (10 μM), MRT68921 (1 μM), AZD8055 (1 μM), or bafilomycin A1 (50 nM) is included.

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

MRT 68921, MRT-68921, MRT68921, ULK, ULK1, ULK2, Unc-51 like kinase

Compound Identifiers

PubChem CID

59225335