MQAE

SKU: orb1304021

Description

MQAE is a fluorescent chloride indicator offering superior sensitivity and selectivity over traditional methods like 36Cl or microelectrodes, as its fluorescence is quenched upon collision with chloride ions. It is widely used in both in vitro and in vivo research to study chloride channel physiology, neuronal inhibition, and cystic fibrosis.

Key Properties

• CAS Number: 162558-52-3
• MW: 326.19
• Purity: 99.81%
• Formula: C₁₄H₁₆BrNO₃
• SMILES: [Br-].CCOC(=O)C[n+]1cccc2cc(OC)ccc12
• Target: Others
• Solubility: 10% DMSO+90% Saline:2.5 mg/mL (7.66 mM);DMSO:62.5 mg/mL (191.61 mM)

Bioactivity

• In Vitro:
  I. Chloride ion detection 1. Solution preparation: Dissolve MQAE in DMSO or aqueous solution. Make sure the concentration of the solution is suitable for cell experiments, generally 1-10 µM. 2. Staining: Add cell culture to medium containing MQAE and incubate for 30 minutes to 1 hour to allow the probe to penetrate into the cells. 3. Fluorescence detection: Use fluorescence microscopy or flow cytometer for detection, with an excitation wavelength of 350 nm and an emission wavelength of 460 nm to monitor the effect of chloride ion concentration on fluorescence intensity. II. pH stability Use MQAE to monitor chloride ion activity under different pH conditions to confirm that it remains stable under low chloride ion concentration conditions. III. Confocal microscopy and flow cytometer Use labeled cells for fluorescence microscopy or flow cytometer experiments. Use a 350 nm UV excitation source and monitor fluorescence emission at 460 nm to detect the effect of changes in chloride ion concentration on the intracellular environment.

Storage & Handling

• Storage: store at low temperature,keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

MQAE, inhibit, Inhibitor

Compound Identifiers

PubChem CID

2762651