MPTP hydrochloride

SKU: orb1303183

Description

MPTP hydrochloride

Research Area

Cell Biology, Neuroscience, Pharmacology & Drug Discovery

Key Properties

• CAS Number: 23007-85-4
• MW: 209.72
• Purity: 99.65%
• Formula: C₁₂H₁₆ClN
• SMILES: Cl.CN1CCC(=CC1)c1ccccc1
• Target: Apoptosis,Dopamine Receptor
• Solubility: 10% DMSO+40% PEG300+5% Tween 80+45% Saline:1 mg/mL (4.77 mM);DMSO:23.86 mg/mL (113.77 mM);H2O:10 mg/mL (47.68 mM)

Bioactivity

• Target IC50:
  Phrenic nerve - diaphragm:53 mM|Phrenic nerve - diaphragm (Pretreatment with 4-phenylpyridine):18 mM
• In Vivo:
  METHODS: To construct a subacute Parkinson's model, MPTP hydrochloride (30 mg/kg in 0.9% saline) was administered intraperitoneally to C57BL/6 mice once daily for five days. RESULTS: Subacute MPTP hydrochloride treatment did not cause significant motor deficits, although the dopaminergic system was severely impaired. mPTP hydrochloride significantly increased the level of α-synuclein and the number of astrocytes in the striatum and disrupted the blood-brain barrier in the substantia nigra pars compacta. METHODS: To study the effects of different models on the behavior and pathology of a mouse model of Parkinson's disease, MPTP hydrochloride was used intraperitoneally to construct a subacute model group and a chronic model group. In the subacute model group, MPTP hydrochloride (30 mg/kg) was administered once daily for ten days. In the chronic model group, MPTP hydrochloride (30 mg/kg) was injected once every 3.5 days for five weeks for ten times. RESULTS: In the MPTP hydrochloride-induced subacute Parkinson's disease mouse model, there was a small loss of dopaminergic neurons in the midbrain, but there was no effect on the behavior.The MPTP hydrochloride-induced chronic Parkinson's disease mouse model lost a large number of dopaminergic neurons, which was accompanied by anxiety-like behaviors in addition to motor dysfunction.
• In Vitro:
  METHODS: Human neuroblastoma cells M17 were treated with MPTP hydrochloride (1-50 μM) for 48 h. The expression levels of target proteins were detected by Western Blot. RESULTS: MPTP hydrochloride promoted Tau protein phosphorylation in M17 cells. METHODS: Neuroblastoma cell N2AB-1 and glioma cell C6 were treated with MPTP hydrochloride (0.33-33.7 μM) for 24 h, and the growth curves were examined. RESULTS: MPTP hydrochloride did not affect the cell number of actively growing N2AB-1 or C6 cells.

Storage & Handling

• Storage: keep away from moisture,keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

penetrant, Dopamine, Dopamine Receptor, DopamineReceptor, Disease, brain, Apoptosis, inhibit, MPTP, MPTP hydrochloride, MPTP Hydrochloride, MPTP-hydrochloride, neurotoxin, Parkinson's, Inhibitor

Compound Identifiers

PubChem CID

161406