Mouse X-Ray Repair Cross Complementing 5 (XRCC5) ELISA Kit

SKU: orb780854

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to X-Ray Repair Cross Complementing 5(XRCC5).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse XRCC5. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse XRCC5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse XRCC5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse XRCC5 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Mouse
• Sample Types: tissue homogenates, cell lysates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.16-10 ng/mL
• Sensitivity: 0.057 ng/mL
• Concentration: 10 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

KARP1 ELISA Kit, KU80 ELISA Kit, KUB2 ELISA Kit, Ku86 ELISA Kit, NFIV ELISA Kit, TLAA ELISA Kit, Lupus Ku Autoantigen Protein P80 ELISA Kit, Nuclear factor IV ELISA Kit, 86 kDa of Ku antigen ELISA Kit, CTC box-binding factor 85 kDa ELISA Kit, Thyroid-lupus autoantigen ELISA Kit

Quick Database Links

UniProt

P27641