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Mouse Von Willebrand Factor (vWF) ELISA Kit

Mouse Von Willebrand Factor (vWF) ELISA Kit

Catalog Number: orb2668196

DispatchUsually dispatched within 5-10 working days
$ 300.00
Catalog Numberorb2668196
CategoryAssays and Kits
DescriptionMouse Von Willebrand Factor (vWF) ELISA Kit
UniProt IDQ8CIZ8
Application notesstandard: 10 µg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse vWF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse vWF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse vWF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse vWF in the samples is then determined by comparing the OD of the samples to the standard curve
Assay Time3.5h
Range0.16-10 µg/mL
Sensitivity0.049 µg/mL
Sample Typesplasma
Alternative namesF8VWF; VWD; von Willebrand antigen 2
NoteFor research use only
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