Mouse Visceral Adipose Tissue Derived Serine Protease Inhibitor (Vaspin) ELISA Kit

SKU: orb2667956

Description

Mouse Visceral Adipose Tissue Derived Serine Protease Inhibitor (Vaspin) ELISA Kit

Research Area

Metabolism Research

Images & Validation

• Application Notes:
  standard: 6000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse Vaspin. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse Vaspin. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse Vaspin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse Vaspin in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Mouse
• Sample Types: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 93.75-6000 pg/mL
• Sensitivity: 35.01 pg/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

SERPINA12; OL-64; Visceral Adipose-Specific SERPIN; Serine Proteinase Inhibitor,Clade A Member 12; Visceral adipose-specific serpin

Quick Database Links

UniProt

Q7TMF5