Mouse Vesicular Glutamate Transporter 1 (VGLUT1) ELISA Kit

SKU: orb780903

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Vesicular Glutamate Transporter 1(VGLUT1).

Research Area

Signal Transduction

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse VGLUT1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse VGLUT1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse VGLUT1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse VGLUT1 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Mouse
• Sample Types: Tissue homogenates and other biological fluids.
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.32-20 ng/mL
• Sensitivity: 0.127 ng/mL
• Concentration: 20 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

SLC17A7 ELISA Kit, BNPI ELISA Kit, Solute Carrier Family 17 Member 7,Sodium-Dependent Inorganic Phosphate Cotransporter ELISA Kit, Brain-specific Na(+)-dependent inorganic phosphate cotransporter ELISA Kit

Quick Database Links

UniProt

Q3TXX4