Mouse Ubiquinol-cytochrome c reductase (UQCRFS1) ELISA Kit

SKU: orb782315

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to UQCRFS1(Ubiquinol-cytochrome c reductase).

Research Area

Metabolism Research

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse UQCRFS1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse UQCRFS1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse UQCRFS1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse UQCRFS1 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Mouse
• Sample Types: Tissue homogenates, cell lysates and other biological fluids.
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.16-10 ng/mL
• Sensitivity: 0.094 ng/mL
• Concentration: 10 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

Complex III subunit 5 ELISA Kit, Complex III subunit IX ELISA Kit, Rieske iron sulfur protein ELISA Kit, RIP1 ELISA Kit, RIS1 ELISA Kit, RISP ELISA Kit, UQCR5 ELISA Kit, UQCRFS1 ELISA Kit

Quick Database Links

UniProt

Q9CR68