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Mouse Tumor Protein p53 (TP53) ELISA Kit

Mouse Tumor Protein p53 (TP53) ELISA Kit

Catalog Number: orb2668024

DispatchUsually dispatched within 5-10 working days
$ 300.00
Catalog Numberorb2668024
CategoryAssays and Kits
DescriptionMouse Tumor Protein p53 (TP53) ELISA Kit
Range78.13-5000 pg/mL
Assay Time3.5h
UniProt IDP02340
Sensitivity16.3 pg/mL
Alternative namesp53; LFS1; TRP53; Li-Fraumeni Syndrome; Cellular t
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NoteFor research use only
Application notesstandard: 5000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse TP53. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse TP53. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse TP53, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse TP53 in the samples is then determined by comparing the OD of the samples to the standard curve
Sample TypesSerum, plasma and other biological fluids.
Expiration DatePlease enquire.