Mouse Transforming Growth Factor Beta 2 (TGFb2) ELISA Kit

SKU: orb779074

Description

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Transforming Growth Factor Beta 2(TGFb2) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Transforming Growth Factor Beta 2(TGFb2).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 2000 pg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse TGFb2 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse TGFb2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse TGFb2 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Mouse
• Sample Types: Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids.
• Assay Type: Competitive
• Assay Time: 2.5h
• Range: 31.25-2000 pg/mL
• Sensitivity: 8.95 pg/mL
• Concentration: 2000 pg/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

TGF-B2 ELISA Kit, TGF-Beta2 ELISA Kit, G-TSF ELISA Kit, LAP ELISA Kit, Cetermin ELISA Kit, Polyergin ELISA Kit, Latency-associated peptide ELISA Kit, BSC-1 cell growth inhibitor ELISA Kit, Glioblastoma-derived T-cell suppressor factor ELISA Kit

Quick Database Links

UniProt

P27090