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Mouse Transferrin Receptor (TFR) ELISA Kit

Mouse Transferrin Receptor (TFR) ELISA Kit

Catalog Number: orb2667208

DispatchUsually dispatched within 5-10 working days
$ 300.00
Catalog Numberorb2667208
CategoryAssays and Kits
DescriptionMouse Transferrin Receptor (TFR) ELISA Kit
UniProt IDQ62351
Application notesstandard: 10 µg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse TFR. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse TFR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse TFR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse TFR in the samples is then determined by comparing the OD of the samples to the standard curve
Assay TypeSandwich
Assay Time3.5h
Range0.16-10 µg/mL
Sensitivity0.052 µg/mL
Sample Typesserum, plasma, tissue homogenates and other biological fluids
Alternative namesCD71; TFR1; P90; TFRC; T9; Trfr; sTfR; Transferrin
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NoteFor research use only
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