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Mouse Thyrotropin Releasing Hormone (TRH) ELISA Kit

Catalog Number: orb776506

DispatchUsually dispatched within 5-10 working days
$ 310.00
Catalog Numberorb776506
CategoryAssays and Kits
DescriptionThis assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Thyrotropin Releasing Hormone(TRH) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Thyrotropin Releasing Hormone(TRH).
ReactivityMouse
Concentration500 pg/mL
UniProt IDQ62361
Tested applicationsELISA
Application notesstandard: 500 pg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse TRH protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse TRH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse TRH in the samples is then determined by comparing the OD of the samples to the standard curve
Assay Time2.5h
Range7.82-500 pg/mL
Sensitivity2.69 pg/mL
Sample Typesserum, plasma and other biological fluids
Alternative namesTRF ELISA Kit, Thyrotropin-Releasing Factor ELISA
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NoteFor research use only
Mouse Thyrotropin Releasing Hormone (TRH) ELISA Kit