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Mouse Thyrotropin Releasing Hormone (TRH) ELISA Kit

Catalog Number: orb776506

DispatchUsually dispatched within 5-10 working days
$ 310.00
Catalog Numberorb776506
CategoryAssays and Kits
DescriptionThis assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Thyrotropin Releasing Hormone(TRH) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Thyrotropin Releasing Hormone(TRH).
Tested applicationsELISA
ReactivityMouse
Range7.82-500 pg/mL
Assay Time2.5h
Concentration500 pg/mL
UniProt IDQ62361
Sensitivity2.69 pg/mL
Alternative namesTRF ELISA Kit, Thyrotropin-Releasing Factor ELISA
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NoteFor research use only
Application notesstandard: 500 pg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse TRH protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse TRH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse TRH in the samples is then determined by comparing the OD of the samples to the standard curve
Sample Typesserum, plasma and other biological fluids
Expiration DatePlease enquire.
Mouse Thyrotropin Releasing Hormone (TRH) ELISA Kit