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| Catalog Number | orb775551 |
|---|---|
| Category | Assays and Kits |
| Description | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Thrombospondin 1(THBS1). |
| Tested applications | ELISA |
| Reactivity | Mouse |
| Range | 0.63-40 ng/mL |
| Assay Time | 3.5h |
| Concentration | 40 ng/mL |
| UniProt ID | P35441 |
| Sensitivity | 0.266 ng/mL |
| Alternative names | TSP1 ELISA Kit, Thrombospondin-1p180 ELISA Kit Read more... |
| Note | For research use only |
| Application notes | standard: 40 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse TSP-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse TSP-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse TSP-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse TSP-1 in the samples is then determined by comparing the OD of the samples to the standard curve |
| Sample Types | plasma, tissue homogenates and other biological fluids |
| Expiration Date | Please enquire. |
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- Item 1 of 1
Mouse THBS1 ELISA Kit [orb409475]
Mouse
3.12 ng/ml-200 ng/ml
1.56 ng/ml
48 T, 24 T, 96 T, 10 x 96 T, 5 x 96 T
