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Catalog Number | orb707292 |
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Category | Proteins |
Description | Recombinant Mouse Thrombopoietin Protein is produced by mammalian expression system and the target gene encoding Ser22Thr356 is expressed with a 6His tag at the N-terminus. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Mouse |
Form/Appearance | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
Purity | Greater than 95% as determined by reducing SDS-PAGE. |
Target | THPO |
Entrez | 21832 |
UniProt ID | P40226 |
Protein Sequence | HHHHHHSPVA PACDPRLLNK LLRDSHLLHS RLSQCPDVDP LSIPVLLPAV DFSLGEWKTQ TEQSKAQDIL GAVSLLLEGV MAARGQLEPS CLSSLLGQLS GQVRLLLGAL QGLLGTQLPL QGRTTAHKDP NALFLSLQQL LRGKVRFLLL VEGPTLCVRR TLPTTAVPSS TSQLLTLNKF PNRTSGLLET NFSVTARTAG PGLLSRLQGF RVKITPGQLN QTSRSPVQIS GYLNRTHGPV NGTHGLFAGT SLQTLEASDI SPGAFNKGSL AFNLQGGLPP SPSLAPDGHT PFPPSPALPT THGSPPQLHP LFPDPSTTMP NSTAPHPVTM YPHPRNLSQE T |
Source | Human cells |
Endotoxins | Endotoxin: Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test. |
Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
Buffer/Preservatives | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
Alternative names | MGDF protein, Thrombopoietin protein, C-mpl ligand Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by reducing SDS-PAGE. | |
Human cells |
Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The ED50 range ≤4ng/ml. | |
HEK293 |
Greater than 95.0% as determined by SDS-PAGE. | |
Escherichia Coli |