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Catalog Number | orb2667358 |
---|---|
Category | Assays and Kits |
Description | Mouse Testosterone (Testo) ELISA Kit |
Range | 1.56-100 ng/mL |
Assay Time | 2.5h |
Sensitivity | 0.68 ng/mL |
Alternative names | T; Testesteron; Testeronun Read more... |
Note | For research use only |
Application notes | standard: 100 ng/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse Testo protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse Testo. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse Testo in the samples is then determined by comparing the OD of the samples to the standard curve |
Sample Types | serum, plasma, other biological fluids |
Expiration Date | Please enquire. |
Mouse | |
15.6 pg/ml-1000 pg/ml. | |
4 pg/ml. |