Mouse TAPA1(Target Of The Antiproliferative Antibody 1) ELISA Kit

SKU: orb2999112

Description

Mouse TAPA1(Target Of The Antiproliferative Antibody 1) ELISA Kit

Research Area

Cancer Biology, Immunology & Inflammation

Images & Validation

• Application Notes:
  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse TAPA1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse TAPA1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse TAPA1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse TAPA1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Key Properties

• Reactivity: Mouse
• Sample Types: Serum, plasma, tissue homogenates, cell lysates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 46.88-3000 pg/mL
• Sensitivity: 11.76 pg/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

CD81; S5.7; TAPA1; TSPAN28; Tetraspanin 28; Target Of Antiproliferative Antibody 1; 26 kDa cell surface protein TAPA-1; CD81 antigen

Quick Database Links

UniProt

P35762