Mouse Soluble Fms-Like Tyrosine Kinase Receptor 1 (sFLT) ELISA Kit

SKU: orb1146932

Description

The Mouse Soluble Fms-Like Tyrosine Kinase Receptor 1 (sFLT) ELISA Kit uses a sandwich enzyme immunoassay to quantify Soluble Fms-Like Tyrosine Kinase Receptor 1 (sFLT) in mouse serum, plasma, tissue homogenates, saliva, cell lysates, follicular fluid and other biological fluids. The kit has a detection range of 78.13-5000 pg/mL and a sensitivity of 29 pg/mL.

Research Area

Pharmacology & Drug Discovery

Images & Validation

• Application Notes:
  standard: 5000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse sFLT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse sFLT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse sFLT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse sFLT in the samples is then determined by comparing the OD of the samples to the standard curve

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Key Properties

• Reactivity: Mouse
• Sample Types: serum, plasma, tissue homogenates, saliva, cell lysates, follicular fluid and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 78.13-5000 pg/mL
• Sensitivity: 29 pg/mL
• Concentration: 5000 pg/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Expiration Date: Please enquire.
• Disclaimer: For research use only

Alternative Names

Flt1, Fms-Related Tyrosine Kinase 1, Vascular Permeability Factor Receptor, Fms-like tyrosine kinase 1, Tyrosine-protein kinase receptor FLT